Standard: G-6-PDH-52

Specific Activity: > 250 units/mg protein (at 25°C and pH 8.5)

Preparation: Ammonium Sulfate

Reaction Equation:
D-Glucose-6-Phosphate + NADP⁺ = D-glucono-1,5-lactone-6-phosphate + NADPH + H⁺

Contaminants: (as G-6-PDH activity = 100%)
Gluutathione reductase < 0.2 %
Hexokinase < 0.02 %
Phosphogluconate dehydrogenase < 0.01 %
Phosphoglucose isomerase < 0.01 %
Phosphoglucomutase < 0.01 %
Creatine kinase < 0.001 %
Myokinase < 0.01 %
ATPase < 0.001 %

State: Purified enzyme in solution
One mL of suspension contains the following
Protein 0.5 ~ 20 mg
K-PO₄ 0.01 ~ 0.1 mol/L
(NH₄)₂SO₄ 3.3 mol/L
EDTA¥Na₂ 0.1 ~ 5.0 mmol/L
pH 7.0 ± 0.5

Conditions for Storage: 2 - 10 °C