ProtocatechuateFoxygen 3,4-oxidoreductase (decyclizing) [EC 1.13.11.3]
Host cell : E.coli

Catalyzing Reaction: 3,4-Dihydroxybenzoate + O₂ 3-Carboxy-cis,cis-muconate

Appearance: Solution with preservative

Activity: (at 37°C) > 80 U/mL

Specific Activity: > 3 U/mg protein

Contaminant: (percentage of rPCO activity)
NADPH oxidase < 0.01 %
Alkaline phosphatase < 0.002 %

Storage: 1 - 10°C (do not freeze)

Characteristics:
Gene: Protocatechuate 3,4-dioxygenase gene cloned from Acinetobacter baumannii
Production: Purified from the extract of transformed E.coli by conventional chromatographies
Purity: Single major band on SDS-PAGE (low contaminants from host E.coli)
Application: Useful for removal of protocatechuate derived from choline esterase determination
Stability: Wide range of pH stability from 5 to 10