Specifications ATP: D-hexose-6-phosphotransferase (EC 2.7.1.1) from yeast.
Preparation: Ammonium Sulfate
Specific activity: > 180 U/mg protein (at 25°C and pH 7.5)
Contaminants: (as HK activity = 100%) Phosphoglucose isomerase < 0.003 % Glutathione reductase < 0.005 % Myokinase < 0.001 % Phosphogluconate dehydrogenase < 0.001 % Phosphoglucomutase < 0.001 % Glucose-6-phosphate dehydrogenase < 0.005 % Creatine kinase < 0.005 % ATPase < 0.003 %
State: Highly purified enzyme in solution One mL of suspension contains the following Protein 2 ~ 50 mg K-PO4 0.01 ~ 0.1 mol/L EDTA.Na2 0.1 ~ 5.0 mmol/L (NH4)2SO4 3.0 mol/L pH 7.0 ± 0.5
Conditions for Storage: 2 ~ 10°C
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