Preparation: Lyophilized

Reaction Equation:
L-Glutamate + H₂O + NADP⁺ = 2-oxoglutarate + NH₃ + NADPH

Specific activity: > 10 U/mg protein
(for reduction of α-ketoglutarate to L-glutamate at 25°C and pH 7.6)

Contaminants: (as GlDH activity = 100%)
Glucose-6-phosphate dehydrogenase < 0.1 %
Phosphogluconate dehydrogenase < 0.5 %
Glutamate dehydrogenase (NAD⁺) < 0.1 %
Glutathione reductase < 0.1 %
NADPH oxidase < 0.01 %

State: Ammonium sulfate free, lyophilized powder of highly purified enzyme
One mg of powder contains the following
Protein 700 ± 200 µg
K-PO4 300 ± 300 µg

Condition for Storage: Lyophilized at -20°C.