Bacterial Protocatechuate 3,4-Dioxygenase (rPCO)

Download Datasheets

Catalog Number Pack Size

Catalog Number 46852004

Pack Size 300 units

Catalog Number 46852904

Pack Size 10 ku, 50 ku, 250 ku, 1000 ku

Specifications

Source

Recombinant from Bacteria

EC

1.13.11.3

Product Form

Solution

Specific Activity

> 3 Units / mg

Reaction Equation

3,4-Dihydroxybenzoate + O2 3-Carboxy-cis,cis-muconate

Contaminants

NADPH oxidase < 0.01 % Alkaline phosphatase < 0.002 %

Other Features

Gene: Protocatechuate 3,4-dioxygenase gene cloned from Acinetobacter baumannii
Production: Purified from the extract of transformed E.coli by conventional chromatographies
Purity: Single major band on SDS-PAGE (low contaminants from host E.coli)
Application: Useful for removal of protocatechuate derived from choline esterase determination
Stability: Wide range of pH stability from 5 to 10

Storage Condition

1 - 10°C (do not freeze)

Product Citations

Hsu, C. F., Chang, K. C., Chen, Y. L., Hsieh, P. S., Lee, A. I., Tu, J. Y., ... & Wen, J. D. (2021). Formation of frameshift-stimulating RNA pseudoknots is facilitated by remodeling of their folding intermediates. Nucleic acids research, 49(12), 6941-6957.

Lu, C. H., Yeh, H. Y., Su, G. C., Ito, K., Kurokawa, Y., Iwasaki, H., ... & Li, H. W. (2018). Swi5–Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation. Proceedings of the National Academy of Sciences, 115(43), E10059-E10068.

Senavirathne, G., Mahto, S. K., Hanne, J., O'Brian, D., & Fishel, R. (2016). Dynamic unwrapping of nucleosomes by HsRAD51 that includes sliding and rotational motion of histone octamers. Nucleic Acids Research, gkw920.

Wang, G., Ang, C. E., Fan, J., Wang, A., Moffitt, J. R., & Zhuang, X. (2020). Spatial organization of the transcriptome in individual neurons. BioRxiv.

Zhang, M., Eichhorn, S. W., Zingg, B., Yao, Z., Cotter, K., Zeng, H., ... & Zhuang, X. (2021). Spatially resolved cell atlas of the mouse primary motor cortex by MERFISH. Nature, 598(7879), 137-143.

Wilson, H., Lee, M., & Wang, Q. (2021). Probing DNA-protein interactions using single-molecule diffusivity contrast. Biophysical Reports, 1(1), 100009.

Chen, R., Blosser, T. R., Djekidel, M. N., Hao, J., Bhattacherjee, A., Chen, W., ... & Zhang, Y. (2021). Decoding molecular and cellular heterogeneity of mouse nucleus accumbens. Nature Neuroscience, 24(12), 1757-1771.

Schamber, M. R., & Vafabakhsh, R. (2022). Mechanism of sensitivity modulation in the calcium-sensing receptor via electrostatic tuning. Nature communications, 13(1), 1-14.

Liauw, B. W. H., Foroutan, A., Schamber, M. R., Lu, W., Afsari, H. S., & Vafabakhsh, R. (2022). Conformational fingerprinting of allosteric modulators in metabotropic glutamate receptor 2. Elife, 11, e78982.

Liu, T., Khanal, S., Hertslet, G. D., & Lamichhane, R. (2023). Single-molecule analysis reveals that a glucagon-bound extracellular domain of the glucagon receptor is dynamic. Journal of Biological Chemistry, 105160.

Kumar, A., Schrader, A., Boroojeny, A., Asadian, M., Lee, J., Song, Y., ... & Sinha, S. (2023). Intracellular Spatial Transcriptomic Analysis Toolkit (InSTAnT).

Wintersinger, C. M., Minev, D., Ershova, A., Sasaki, H. M., Gowri, G., Berengut, J. F., ... & Shih, W. M. (2023). Multi-micron crisscross structures grown from DNA-origami slats. Nature Nanotechnology, 18(3), 281-289.

© Copyright 2024. All Rights Reserved.

Privacy | Sitemap