rPCO:
Protocatechuate 3,4-Dioxygenase (rPCO) is used to scavenge oxygen and reduce photobleaching of fluorophores, improving the quality of single-molecule experiments. Use of rPCO in improving the stability of dyes/ fluorophores has provided new insight in the field of single molecule microscopy by increasing the fluorophore half life by 100 fold.
The in vitro diagnostics (IVD) industry demands a reliable supply of high-quality raw materials to ensure the accuracy and consistency of diagnostic tests. OYC Americas specialize in providing these essential components, and a key part of their portfolio includes a range of high-performance enzymes, such as recombinant Protocatechuate 3,4-Dioxygenase (rPCO).
We offer Bacterial Protocatechuate 3,4-Dioxygenase (rPCO) as a product for its clients in the life sciences and diagnostics sectors. As a company that focuses on producing high-purity biochemicals, OYC’s rPCO is an ideal fit for the rigorous requirements of IVD. It is produced through recombinant methods, typically expressed in E. coli, and then purified using conventional chromatography. This ensures a product that has high specific activity and is free from unwanted contaminants, a common problem with enzymes isolated from their natural sources.
rPCO’s Role in Diagnostic Applications
The OYC Americas rPCO highlight its specific applications in diagnostics, linking the enzyme’s unique properties to the needs of the IVD industry. Key applications include:
- Oxygen Scavenging for Fluorescence-Based Assays: The data notes that the enzyme is “used to improve organic fluorophore-stability in single-molecule experiments.” This application is critical for highly sensitive fluorescent diagnostic platforms. By providing an efficient and clean method to remove oxygen from the reaction environment, OYC Americas’ rPCO helps developers and researchers create more robust and stable assays. This is especially important for next-generation diagnostic tools that rely on single-molecule detection for extreme sensitivity.
By providing a high-quality, recombinant version of PCO, OYC Americas supports the development and manufacturing of advanced diagnostic assays. This product meets the technical specifications required for sensitive applications and represents a move towards more sustainable and scalable production methods, aligning with the evolving needs of the global IVD market. In an industry where precision and reliability are paramount, OYC’s rPCO is a specialized and essential component for advanced diagnostic solutions.
Click the link below for the product information:
https://www.oycus.com/product/bacterial-protocatechuate-34-dioxygenase-rpco/